Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.)

Objective:To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum).Methods:For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The α-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate.Results:The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ -terpinene (12.96%), 4-terpenyl acetate (12.18%) and α -pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (ICConclusions:Observed antioxidant and α-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes.

Antioxidant and glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.)

Objective: To investigate the antioxidant and glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus Niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ -terpinene (12.96%), 4-terpenyl acetate (12.18%) and pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (IC